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Interactions and Translational Dynamics of Phosphatidylinositol Bisphosphate (PIP2) Lipids in Asymmetric Lipid Bilayers

Shi, Xiaojun ; Kohram, Maryam ; Zhuang, Xiaodong ; Smith, Adam W

Langmuir : the ACS journal of surfaces and colloids, 23 February 2016, Vol.32(7), pp.1732-41 [Rivista Peer Reviewed]

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  • Titolo:
    Interactions and Translational Dynamics of Phosphatidylinositol Bisphosphate (PIP2) Lipids in Asymmetric Lipid Bilayers
  • Autore: Shi, Xiaojun ; Kohram, Maryam ; Zhuang, Xiaodong ; Smith, Adam W
  • Note di contenuto: Phosphatidylinositol phosphate (PIP) lipids are critical to many cell signaling pathways, in part by acting as molecular beacons that recruit peripheral membrane proteins to specific locations within the plasma membrane. Understanding the biophysics of PIP-protein interactions is critical to developing a chemically detailed model of cell communication. Resolving such interactions is challenging, even in model membrane systems, because of the difficulty in preparing PIP-containing membranes with high fluidity and integrity. Here we report on a simple, vesicle-based protocol for preparing asymmetric supported lipid bilayers in which fluorescent PIP lipid analogues are found only on the top leaflet of the supported membrane facing the bulk solution. With this asymmetric distribution of lipids between the leaflets, the fluorescent signal from the PIP lipid analogue reports directly on interactions between the peripheral molecules and the top leaflet of the membrane. Asymmetric PIP-containing...
  • Fa parte di: Langmuir : the ACS journal of surfaces and colloids, 23 February 2016, Vol.32(7), pp.1732-41
  • Soggetti: Lipid Bilayers -- Chemistry ; Phosphatidylinositol Phosphates -- Chemistry
  • Lingua: Inglese
  • Tipo: Articolo
  • Identificativo: E-ISSN: 1520-5827 ; PMID: 26829708 Version:1 ; DOI: 10.1021/acs.langmuir.5b02814

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