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Patterned two-photon photoactivation illuminates spatial reorganization in live cells

Smith, Adam W ; Smoligovets, Alexander A ; Groves, Jay T

The journal of physical chemistry. A, 28 April 2011, Vol.115(16), pp.3867-75 [Rivista Peer Reviewed]

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  • Titolo:
    Patterned two-photon photoactivation illuminates spatial reorganization in live cells
  • Autore: Smith, Adam W ; Smoligovets, Alexander A ; Groves, Jay T
  • Note di contenuto: Photoactivatable fluorescent proteins offer the possibility to optically tag and track the location of molecules in their bright state with high spatial and temporal resolution. Several reports of patterned photoactivation have emerged since the development of a photoactivatable variant of the green fluorescent protein (PaGFP) and the demonstration of two-photon activation of PaGFP. To date, however, there have been few methods developed to quantify the spatial reorganization of the photoactivated population. Here we report on the use of singular value decomposition (SVD) to track the time-dependent distribution of fluorophores after photoactivation. The method was used to describe live-cell actin cytoskeleton behavior in primary murine T-cells, in which a dynamic cytoskeleton is responsible for the reorganization of membrane proteins in response to antigen peptide recognition. The method was also used to observe immortalized simian kidney (Cos-7) cells, in which the cytoskeleton is more...
  • Fa parte di: The journal of physical chemistry. A, 28 April 2011, Vol.115(16), pp.3867-75
  • Soggetti: Photons ; Green Fluorescent Proteins -- Chemistry ; T-Lymphocytes -- Chemistry
  • Lingua: Inglese
  • Tipo: Articolo
  • Identificativo: E-ISSN: 1520-5215 ; PMID: 21391691 Version:1 ; DOI: 10.1021/jp108295s

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