skip to main content
Lingue:

Amino-functionalized DNA: the properties of C5-amino-alkyl substituted 2'-deoxyuridines and their application in DNA triplex formation

Brazier, John A ; Shibata, Takayuki ; Townsley, John ; Taylor, Brian F ; Frary, Elaine ; Williams, Nicholas H ; Williams, David M

Nucleic acids research, 2005, Vol.33(4), pp.1362-71 [Rivista Peer Reviewed]

Fulltext disponibile

Vedi tutte le versioni
Citazioni Citato da
  • Titolo:
    Amino-functionalized DNA: the properties of C5-amino-alkyl substituted 2'-deoxyuridines and their application in DNA triplex formation
  • Autore: Brazier, John A ; Shibata, Takayuki ; Townsley, John ; Taylor, Brian F ; Frary, Elaine ; Williams, Nicholas H ; Williams, David M
  • Note di contenuto: The incorporation of C5-amino-modified 2'-deoxyuridine analogues into DNA have found application in nucleic acid labelling, the stabilization of nucleic acid structures, functionalization of nucleic acid aptamers and catalysts, and the investigation of sequence-specific DNA bending. In this study, we describe the physicochemical properties of four different C5-amino-modified 2'-deoxyuridines in which the amino group is tethered to the base via a 3-carbon alkyl, Z- or E-alkenyl or alkynyl linker. Conformational parameters of the nucleosides and their pK(a) values were deduced using 1H NMR. All of them display the expected anti-conformation of the nucleoside with 2'-endo sugar puckers for the deoxyribose ring. A preference for the cisoid conformation for the Z-alkenyl analogue is found, while the E-alkenyl analogue exists exclusively as its transoid conformation. The pK(a) values range from 10.0 for the analogue with an aliphatic propyl linker to 8.5 for the propargylamino analogue. The analogues...
  • Fa parte di: Nucleic acids research, 2005, Vol.33(4), pp.1362-71
  • Soggetti: Amines -- Chemistry ; DNA -- Chemistry ; Deoxyuridine -- Analogs & Derivatives
  • Lingua: Inglese
  • Tipo: Articolo
  • Identificativo: E-ISSN: 1362-4962 ; PMID: 15745996 Version:1

Ricerca in corso nelle risorse remote ...