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Sulfite species enhance carbon monoxide release from CO-releasing molecules: Implications for the deoxymyoglobin assay of activity

Mclean, Samantha ; Mann, Brian E ; Poole, Robert K

Analytical Biochemistry, 01 August 2012, Vol.427(1), pp.36-40 [Rivista Peer Reviewed]

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  • Titolo:
    Sulfite species enhance carbon monoxide release from CO-releasing molecules: Implications for the deoxymyoglobin assay of activity
  • Autore: Mclean, Samantha ; Mann, Brian E ; Poole, Robert K
  • Note di contenuto: Carbon monoxide-releasing molecules (CO-RMs) emulate the beneficial (e.g., anti-inflammatory) effects of CO in biology. CO release from CO-RMs is routinely determined in the presence of reduced deoxy-myoglobin by measuring the formation of carboxy-myoglobin (Mb-CO). Previous studies have highlighted discrepancies between the apparent CO release rates of some CO-RMs established using this assay versus other experimental data where a slower or more complex mechanism of release is suggested. It has been hypothesized that some CO-RMs require a CO acceptor, believed to be reduced myoglobin in Mb-CO assays, in order to facilitate the release of CO. Here, we show, for the first time, that CO is not liberated from the ruthenium (Ru)-based [Ru(CO)3Cl2]2 (CORM-2) and [Ru(CO)3Cl(glycinate)] (CORM-3) at an appreciable rate in the presence of reduced myoglobin alone. Rather, we confirm that it is the reducing agent sodium dithionite that facilitates release of CO from these CO-RMs. Other sulfite...
  • Fa parte di: Analytical Biochemistry, 01 August 2012, Vol.427(1), pp.36-40
  • Soggetti: CO-Releasing Molecules ; Myoglobin ; Hemoglobin ; Carbon Monoxide ; Dithionite ; CO-Releasing Molecules ; Myoglobin ; Hemoglobin ; Carbon Monoxide ; Dithionite ; Chemistry ; Anatomy & Physiology
  • Lingua: Inglese
  • Tipo: Articolo
  • Identificativo: ISSN: 0003-2697 ; E-ISSN: 1096-0309 ; DOI: 10.1016/j.ab.2012.04.026

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